Establishment of Loop-mediated Isothermal Amplification (LAMP) Method for Campylobacter jejuni Detection
- VernacularTitle:空肠弯曲菌LAMP快速检测方法的建立
- Author:
Chao LIN
;
Cheng-Zhu LIANG
;
Biao XU
;
Min SUN
;
Cai-Xia LIU
;
Hong-Wei GAO
;
- Publication Type:Journal Article
- Keywords:
Loop-mediated isothermal amplification, Campylobacter jejuni, Detection
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A rapid LAMP detection method with primers designed on genus-specific region identified in the gyrA gene was established in this assay. All four Campylobacter jejuni from different sources were detected positive and fourteen non Campylobacter bacteria were negative, which shows excellent specificity of the primers. Compared with plate count and PCR method, the LAMP method and the PCR method had equal sensitivity, which were three orders of magnitude higher than plate count. In this assay, we also found out that the treatment of DNase could reduce the dead bacteria DNA effectively. The LAMP detection on chicken indicated relatively good result on detection of Campylobacter jejuni combining with treatment of DNase.
