The Apoptosis Induction of CT 26 Cells by Recombinant Clostridium difficile Toxin B
- VernacularTitle:重组艰难梭菌毒素B对小鼠结肠癌CT26细胞的诱导凋亡作用
- Author:
Min CHEN
;
Yan-Fen CAO
;
Shan LI
;
Li-Hua SUN
;
Ting-Ting LIU
;
Ju-Fang WANG
;
- Publication Type:Journal Article
- Keywords:
Clostridium difficile, Recombinant toxin B, Apoptosis, CT26 cells
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the induction of apoptosis of mouse colonic adenocarcinoma CT26 cells by recombinant Clostridium difficile toxin B (rTcdB), CT26 cells were exposed to different concentrations of rTcd B. Inhibition of cell proliferation was measured by MTT assay. The activation of Caspase 3 was measured by colorimetric method. Cell morphological analysis and flow cytometry were performed to confirm cell apoptosis. rTcd B inhibited the proliferation of CT26 cells in a timeand dose-dependent manner. Caspase 3 activity in CT26 cells was elevated remarkably after rTcd B exposure for 6 h, 12 h, 18 h or 24 h, as compared with the control group. Morphological changes were observed by fluorescence microscopy. The exposure of rTcd B to CT26 cells induced a timeand dose-dependent apoptotic cell death as determined by flow cytometry analysis. The results showed that recombinant Clostridium difficile toxin B induced apoptosis of CT26 cells.
