Breeding of Yeast Fusant for Efficient Ethanol Fermentation from Xylose
- VernacularTitle:高效发酵木糖生产乙醇酵母菌株的构建
- Author:
Jie LI
;
Fan LI
;
Chen-Guang LIU
;
Jian-Gang REN
;
Xin-Qing ZHAO
;
Xue-Meng GE
;
Feng-Wu BAI
;
- Publication Type:Journal Article
- Keywords:
Candida shehatae Saccharomyces cerevisiae Protoplast fusion Ethanol fermentation
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Yeast strains with improved ethanol yield are important for efficient bioconversion of lignocellulosic biomass for fuel ethanol.Candida shehatae CICC1766 was adapted to 4%(v/v)ethanol,and then subjected to UV mutagenesis.One respiration deficient mutant Rd-5 with improved xylose fermentation capability was selected.Protoplasts of Rd-5 were inactivated by UV treatment,followed by the PEG-mediated protoplast fusion with a Saccharomyces cerevisiae strain with good ethanol-fermenting capability.The xylose fermenting capability of the fusants was investigated,and the fusant F6 demonstrated good ethanol fermentation performance,producing 18.75g/L ethanol from 50g/L xylose with an ethanol yield of 0.375 or 73.4% of its theoretical value of 0.511.Comparing with its parent Candida shehatae strain,the ethanol yield of F6 was increased by 28%.
