Expression of XYL1 of Candida tropicalis in Pichia pastoris and Xylitol Fermentation by Immobilizing Cells
- VernacularTitle:热带假丝酵母XYL1在Pichia pastoris中的表达及固定化细胞发酵生产木糖醇
- Author:
Jing LI
;
Xuan HE
;
Fei-Wei ZHANG
;
Yan-Qiu SU
;
Hong WANG
;
Fang-Hui LU
;
Tie-Lin CHEN
;
Yi CAO
;
Dai-Rong QIAO
- Publication Type:Journal Article
- Keywords:
XYL1 Candida tropicalis Pichia pastoris Immobilized cells Xylitol
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
XYL1 gene,which encodes xylose reductase with dual coenzyme activity from Candida tropicalis,was transformed into Pichia pastoris X-33 by expression vector pGAPZB.The recombination strain was immobilized in Ca-alginate beads and fermentation characterization is studied using corn cob hydrolysates.Fermentation conditions were as follow:initial pH value 6.0,30℃,initial cell concentration of 20%,the Liquid volume of 28%,rotation speed 130r/min.The average xylitol yield was 37.5% on the optimum condition.This result is expected to provide a new alternative method for producing xylitol on a large scale by bioconversion.
