Rapid Detection of Total Bacterial Number Using NADH Fluorescence Method
- VernacularTitle:NADH荧光法快速检测细菌总数
- Author:
Jing WANG
;
Jing-Xue WANG
;
Hong LIN
;
Ce-Xia MEI
;
- Publication Type:Journal Article
- Keywords:
NADH,Fluorescence,Total bacterial number,Rapid detection
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To set up a new method of detecting bacterial number in situ,NADH fluorescence method based on the fluorescent characteristic of NADH was used.When the concentration of NADH ranged from 10 nmol/L to 0.2 mmol/L,its concentration had a good line relationship to the fluorescence intensity(R2= 0.9905).Separating bacterial cells by centrifugation and extracting NADH with hot Tris-HCl buffer,the re-sult of bacterial count detected with NADH standard plot was 1?104 CFU/mL in an hour.In summary,NADH fluorescence method is rapid,sensitive,simple and reliable to detect total bacterial number.There-fore,the method can be widely applied in the field of food sanitation and safety,environment detection and so on.
