Identification and Simulation Mutation of a High-productive Strain of Poly(?-glutamic acid) Independent of Glutamic Acid
- VernacularTitle:一株非谷氨酸依赖型聚?-谷氨酸高产菌株的鉴定与诱变育种
- Author:
Xiu-Lin SHU
;
Qing-Shan SHI
;
Jing FENG
;
You-Sheng OUYANG
;
Yi-Ben CHEN
;
- Publication Type:Journal Article
- Keywords:
Poly(?-glutamic acid) [?-PGA],Identification,Mutagenic screening,Bacillus licheniformis,Independent-glutamic Acid
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A high productive poly ?-glutamic acid(?-PGA) strain PGA-N in a culture medium containing no L-glutamine was isolated from fermentation products.With the following identifications of colony mor-phology,physiological and biochemistry experiments,and genetics,the strain PGA-N was classified as a Bacillus licheniformis.According to the product environment,the base culture medium having no L-glutamine was simulated.In order to enhance the production of the strain PGA-N,the fermentation condi-tions,such as carbon source,nitrogen source,were optimized and the ?-glutamic acid production reached 5.16 g/L after getting the optimum formulation of this culture medium.PGA-N was mutagenized with com-bination of NTG and UV.A mutant PGA-N-C10 was screened which PGA production was increased from 5.16 g/L to 8.82 g/L.The study also investigated the effects of agitation speed on the cell biomass,?-PGA production and the ?-PGA molecular weight.The ?-PGA yield of PGA-N-C10 was as high as 11.00 g/L when the agitation speed was 400 r/min.
