Optimization of Fermentation Medium for S-adenosylmethionine Production by Candida sp.YQ5
- VernacularTitle:假丝酵母YQ5产S-腺苷甲硫氨酸的发酵培养基优化
- Author:
Fang-Lan GE
;
Sheng YE
;
Gui-Ying CHEN
;
Wei LI
;
Ke WU
;
Liang-Jun DU
;
- Publication Type:Journal Article
- Keywords:
Candida sp.,S-Adenosylmethionine,Fermentation medium
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The optimized cultural medium of fermentation for Candida sp.mutant strain YQ5 to produce S-adenosylmethionine was studied.The results of single factor experiment showed that the most favorable pH value,carbon source,nitrogen source organic nutrient is 6.0,8% sucrose,1.5% tryptone and 2% yeast extract,respectively.As to inorganic salts,MgSO4?7H2O,CaCl2,FeSO4?7H2O,CoCl2,CuSO4?5H2O,H3BO3 could improve accumulation of the intercellular SAM.The ingredients of the culture medium are also opti-mized by the orthogonal experiment.Fermentation for 48 h under the optimal condition resulted in AdoMet production at 1740.0 mg/L.
