Analysis on RAPD Genetic Polymorphism of HIV Related Oral Candida Species
- VernacularTitle:HIV相关性口腔念珠菌RAPD多态性分析
- Author:
You-Cong WU
;
Li BAI
;
You-Hua YUAN
;
Li-Jun GUO
;
- Publication Type:Journal Article
- Keywords:
Genetic polymorphism,Susceptibility,Candida albicans,HIV
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
60 Candida strains were analyzed with randomly amplified polymorphic DNA(RAPD) from HIV-infected patients' oral cavity.The results showed that RAPD-PCR fingerprinting appeared from zero to five bands whose size rang from 300 bp to 2 kb with P2 primer,Candida albicans have three characteristic bands of 300 bp,400 bp and 600 bp in size,non-Candida albicans have the similar bands.They can be clas-sified five genetic clusters and 14 genotypes by Cluster Analysis,two Candida albicans(P385&P403) with fluorocytosine-resistance were classified into C1 genotype for "Squared Euclidean Distance" being 0.115,one Candida albicans(P321) and one Candida glabrata(P522) who were resistant against Amphotericin B were classified into D1 genotype for "Squared Euclidean Distance" being 0.221.So,an abundant genetic polymorphism appeared in Candida species related HIV,RAPD could be as a reference for genotype of Candida albicans;different Candida speices had some special bands,different Candida strains had similar bands in the same species;it is perhaps that some genotypes were relevant to drug resistance.
