Expression of Nanog Gene with the Mediation of Ientiviral Vector in Mouse ES Cells
- VernacularTitle:慢病毒介导Nanog基因在小鼠ES细胞的表达
- Author:
Hong-Guo CAO
;
Hui-Qun YIN
;
Wei-Qin ZHANG
;
Tao CHEN
;
Wei-Ling HUANG
;
- Publication Type:Journal Article
- Keywords:
Mouse Embryonic stem cell Nanog gene Overexpression
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to further study mouse embryonic stem cells(ES cells),lentiviral vector PLL-IRES-Nanog-Neo was constructed.Mouse ES cells overexpressed nanog by mediation of lentiviral were cultured on mouse fetal fibroblast feeders after 2 weeks under G418 media and examined according to gowth characteristics. Results were showed that 918 bp nanog fragments were expressed in mouse ES cells mediated by lentiviral vector PLL-IRES-Nanog-Neo,mouse nanog-ES cells were taken on mass-like image and positve with alkaline phosphatase staining and Oct4 and SSEA1 immunocytochemistry under no LIF condition in the media. It is concluded that mouse ES cells Elevated nanog gene expression by mediation of lentiviral were constucted and cultured.
