Study on Mutated DHDPS Gene as Selectable Marker of Transgenic Plant
- VernacularTitle:DHDPS突变基因作为转基因植物筛选标记的研究
- Author:
Hui-Yong JIA
;
Jia TIAN
;
Pei-Qing LI
;
Jie LI
;
- Publication Type:Journal Article
- Keywords:
Transgenetic plant Selectable marker Biosafety
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The rodipicolinate synthase gene was cloned from Nicotiana tabacum L.cv,which encodes the key enzyme in the synthesis of lysine,and then a vitro molecular reform reliefing feedback suppression was made. Some resistant seedlings were acquired using the mutated gene as selectable marker and analog of lysine as selectable reagent,and the detection results were positive by means of PCR and Real-Time PCR,however the phenotype is somewhat abnormal.
