Cloning and Transformation of lba Gene of Glycine max into Chloroplast of Chlamydomonas reinhardtii
- VernacularTitle:豆血红蛋白基因lba的克隆及其转化衣藻叶绿体
- Author:
Guang-Yu YAN
;
Xiao-Lei LIU
;
Quan-Xi WANG
;
Shuang-Xiu WU
;
- Publication Type:Journal Article
- Keywords:
Chlamydomonas reinhardtii Leghemoglobin Bio-hydrogen production Chloroplast transformation
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To decrease the oxygen content in the cell is a key method to improve hydrogen production in Chlamydomonas reinhardtii.A new approach was developed by transforming the leghemoglobin gene lba,which has high affinity to oxygen,into the chloroplast of C.reinhardtii to get a low dissolved oxygen in the cell and result into improvement of H2 ase activity and H2 yield. The results showed that lba was successfully transformed into the chloroplast of C.reinahrdtii strain 849 and did not affect its growth significantly. The work paved the road for further regulation of lba expression in the chloroplast to improve of hydrogen production of C.reinahrdtii.
