Creation and Characteristics of HepG2/mdr1 Cell Line
- VernacularTitle:HepG2/mdr1稳定细胞系的建立及特性研究
- Author:
Ju ZHANG
;
Jie CHENG
;
Jing CHEN
;
Juan YI
;
Jing SUN
;
Hu-Lai WEI
;
- Publication Type:Journal Article
- Keywords:
Multidrug resistance Human hepatocellular carcinoma Transfection Recombinant plasmid pCI-neo-mdr1
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Eukaryotic expression plasmid pCI-neo-mdr1 which contains human multidrug resistance gene 1(mdr1),was constructed and transferred into human hepatocarcinoma HepG2 cells by use of liposome. G418 was used to screen the cells successfully with mdr1 and the selected cells was named HepG2/mdr1 Morphological and biological properties of HepG2/mdr1 cells were observed. The results show that the constructed HepG2/mdr1 cell line was high efficient and stationary in the expression of mdr1. The work was valuable and desirable for the establishment of multidrug resistant cell models,and for the study of MDR in human hepatoma. Furthermore,the work also provided a perfect model for the research of relationship between insulin resistance and MDR in hepatocarcinoma cells.
