Mn(Ⅱ) Oxidation and Removal by a Manganese-oxidizing Bacterium Bacillus sp. MK3-1
- VernacularTitle:锰氧化菌Bacillus sp. MK3-1的Mn(Ⅱ)氧化特性和除锰能力研究
- Author:
Yan-Jun LIU
;
Jing-Xiao ZHOU
;
Ge-Jiao WANG
;
- Publication Type:Journal Article
- Keywords:
Manganese-oxidizing bacteria, Bacillus, Mn(II)-oxidation, Mn removal, Embedding, Scan elec- tron microscope
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Manganese-oxidizing microorganisms are able to oxidize soluble Mn(II) into insoluble Mn oxides. Such microorganisms are very useful in treatment of Mn-contaminated water. In this research, a Mn(II)- oxidizing bacterium Bacillus sp. MK3-1 was isolated from Mn-contaminated soil. This bacterium has high MnCl2 resistance with a MIC of 20 mmol/L. The results showed that it is able to oxidize and remove more than 96% of Mn(II) in the culture medium. The immobilized solid-embedding Bacillus sp. MK3-1can re- moved 87.12% of manganese contaminated water. The final concentration of MnCl2 after the treatment reached the national discharge standard level. Scan electron microscope observation showed that the pro- duced Mn oxides located on the cell surfaces of Bacillus sp. MK3-1. Energy dispersive spectrdmeter analy- sis indicated that the content of manganese of cell surfaces of Bacillus sp. MK3-1 was 19.60% (W/W). At last we amplified a 903 bp multicopper oxidase gene mnxG encoding the putative Mn(II)-oxidizing protein.The product of mnxG showed 86% identity to the reported multicopper oxidase.
