Isolation and Characterization of a Moderately Halophilic Bacterium High-producing Esterase
- VernacularTitle:一株高产酯酶中度嗜盐菌的分离、鉴定及酯酶部分酶学性质的研究
- Author:
Wei DONG
;
Li-Zhong GUO
;
Cui-Cui LI
;
Lin WANG
;
Wei-Dong LU
;
- Publication Type:Journal Article
- Keywords:
Moderately halophilic bacterium,Idiomarina,Esterase,16S rRNA
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A moderately halophilic bacterium strain DF-B6 capable of degrading Tween 20 was isolated from solar saltern brine by plate method.Based on the phenotype,physiological and biochemical character-istics,and the phylogenetic analysis of 16S rDNA sequence,strain DF-B6 was identified as Idiomarina.The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase,and not a li-pase.The maximum esterase activity was observed in 8% NaCl concentration at 50?C,pH 8.0.The effect of various divalent cations was studied on the activity of esterase from Idiomarina sp.DF-B6 at the concentra-tion of 10 mmol/L,Mg2+,Ca2+ and Mn2+enhanced the esterase activity,while Zn2+,Fe3+ and Cu2+ inhibited its activity.
