Establishment of Two-dimensional Electrophoresis System for Proteome of Monascus ruber
- VernacularTitle:红色红曲菌蛋白质组双向凝胶电泳体系的建立
- Author:
Chen WAN
;
Yang XU
;
Yan-Ping LI
;
Hong-Fei JI
;
- Publication Type:Journal Article
- Keywords:
Monascus ruber Two-dimensional electrophoresis Proteomics
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Two-dimensional electrophoresis(2-DE) is an important technique in proteomics research.The 2-DE system for proteome of Monascus ruber was established by comparing and analyzing the infection caused by different kinds of mediums,lysis buffer and the condition of rehydration.By cultivating the Monascus ruber with YES for 6 days,extracting total protein by TCA-acetone,lysis buffer with 8 mol/L urea,2 mol/L thiourea,4 % CHAPS,1 % DTT and 2 % Bio-lyte,an ideal 2-DE map with higher resolution and better legibility was obtained,which laid a foundation for the further studies on proteome of Monascus ruber.
