Preliminary Discussion on the Structure of a Novel Housefly Pupae Lectin
- VernacularTitle:一种新型家蝇蛹凝集素结构的初步探讨
- Author:
Xiao-Hong CAO
;
De-Zhi MAO
;
Cheng-Jian XU
;
Mei-Fang LU
;
Chao-Long HAN
;
Xiao-Ye WANG
;
Zhe HUO
;
Chun-Ling WANG
;
- Publication Type:Journal Article
- Keywords:
Lectin Glycoprotein Oligosaccharide chain O-glycoside bond N-terminal blocked
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to provide plenty of information about the relationship between its structure and function,the structure of a novel housefly pupae D-galactose binding lectin with the molecular weight 55kDa and immune acitivity was analyzed preliminarily.In the first place,oligosaccharide chain was confirmed to be existed in this kind of novel housefly pupae lectin by the method of gel staining,and then its structure was analyzed with the help of protein sequencing instrument,spectrophotometer color contrast,?-elimination reaction,infrared spectroscopy and atomic force microscopy.This kind lectin was a global-shaped monomer with the diameter 75 nm or so and the protein and oligosaccharide content 97.36% and 2.1% respectively.Peptide chain and oligosaccharide chain was linked by O-glycoside bond with the N-terminal blocked and the sugar ring alpinum type.All above was the reliable theory for further analysis of structure.
