The Study of Enzymatic Activity of Mutant Strain Rhizopus oryzae with L-Malic Acid Accumulation
- VernacularTitle:积累L-苹果酸的米根霉突变株酶活性初探
- Author:
Hao HE
;
Shuang LI
;
Qing XU
;
Yong-Qian FU
;
He HUANG
;
- Publication Type:Journal Article
- Keywords:
Rhizopus oryzae, L-Malic acid, Fumarase, Pyruvate carboxylase (PC), Alcohol dehydrogenase (ADH)
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A stable and efficient L-Malic acid accumulation mutant strain Rhizopus oryzae ME-M15 was discovered occasionally in the mutation breading for fumaric acid producers. Rhizopus oryzae ME-M15 gave a L-Malic acid output of 16.3 g/L on average after fermentation for 96 hours, more than 3 times than that of the parent strain ME-F10. In addition, other metabolites such as ethanol and fumaric acid were re-markably decreased in accordance with the depressed activity of the cytosolic isoenzyme of fumarase and alcohol dehydrogenase in strain ME-M15, while the activity of the pyruvate carboxylase had no significant difference.
