Optimization of an Antimicrobial Peptide R-1 Production by Brevibacillus laterosporus Lh-1
- VernacularTitle:海洋侧孢短芽孢杆菌Brevibacillus laterosporus Lh-1产抗菌肽R-1的培养条件优化
- Author:
Yuan ZHENG
;
Yue-Jun WANG
;
Mi SUN
;
- Publication Type:Journal Article
- Keywords:
Brevibacillus laterosporus Lh-1, Antimicrobial peptide R-1, Plackett-Burman (PB) design, Re- sponse surface methodology(RSM)
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To improve and optimize marine antimicrobial peptide R-1 production by a newly isolated Brevibacillus laterosporus Lh-1, Plackett-Burman (PB) design and response surface methodology (RSM) using central composite design was adopted in culture conditions. MINITAB 15.0 was used for planning the experiments, data analysis, contour diagrams and response optimizations. In this study, PB design was undertaken to evaluate the effects of the fifteen factors. By the statistical regression analysis, the significant factors affecting the novel antimicrobial peptide R-1 in submerged fermentation by Br. laterosporus Lh-1 were determined as follows: glucose, peptone and CaCl2. Then a RSM was used to optimize the above critical internal factors, and the optical concentration of the variables were deter-mined as: 15.72 g/L glucose, 6.01 g/L peptone and 3.29 g/L CaCl2. The content of R-1 was increased from 82.15 kU/mL to 116.27 kU/mL.
