Prokaryotic Expression and Characteristics Reseach of Pinellia Ternate and Pinellia Pedatisecta Lectins
- VernacularTitle:三叶半夏和掌叶半夏凝集素原核表达及特性研究
- Author:
Jiang-Li LIANG
;
Bo CHEN
;
Xiao-Ping TIAN
;
Xiao-Chuan LIU
;
- Publication Type:Journal Article
- Keywords:
Pinellia ternate Pinellia pedatisecta Lectin Prokaryotic expression Hemagglutinating activity
- From:
China Biotechnology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Pinellia ternate and Pinellia pedatisecta lectins with biological functions could be obtained through procaryon expression.Their biological similarities and differences were analyzed and further experiments were conducted to discuss the agglutinative activity difference between polymer lectin and metamer lectin.The results showed that agglutinative activity of Pinellia pedatisecta was four times more than that of Pinellia ternate.The differences of the agglutinative activity and pharmacologic action were mainly because two site mutations of Pinellia pedatisecta in the third active site compared with the amino acids sequence in Pinellia ternate.In addition,the metamer lectin expressed by prokaryotic expression system did not agglutinate rabbit red blood cells.It conduct further discussion about the difference between two lectins and the difference between polymer lectin and metamer lectin.It would be applicable for solving the problem of lacking Pinellia resources.
