Akt1 Silencing Inhibits Lung Metastasis of Murine Breast Cancer Cells
- VernacularTitle:Akt1基因沉默降低小鼠乳腺癌细胞的肺转移能力
- Author:
Hai-Yan LIU
;
Yu-Chao GU
;
Wen-Yi MI
;
Wen-Gong YU
;
- Publication Type:Journal Article
- Keywords:
Akt1 Breast cancer Migration Tumor metastasis
- From:
China Biotechnology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Akt1 is a serine-threonine protein kinase that has been implicated in the control of cellular metabolism,survival and growth.Elevated expression of Akt1 has been noted in a significant percentage of human tumors,promoting cellular metastasis.Conversely,some studies have revealed hyperactivated Akt1 inhibited the invasiveness and metastasis of breast cancer cells.To clarify the definite effect of Akt1 on tumorigenesis and development,Akt1 was silenced by RNAi in the highly metastatic murine breast cancer 4T1 cells.Akt1 silencing didn't affect the proliferation of breast cancer cells in MTT assay,while reduced the migration in Transwell assay.Consistent with the above results,Akt1 silencing didn't change the primary tumor weight,but significantly suppressed lung metastasis of 4T1 cells.These observations indicated Akt1 plays an important role in murine breast cancer metastasis,and suggested that Akt1 might be a therapeutic target for breast cancer metastasis.
