Rapid Construction of RNA Silencing Virus Resistance Vector Containing TMV and CMV Virus-derived Genes
- VernacularTitle:烟草花叶病毒和黄瓜花叶病毒双价RNA沉默抗病载体的快速构建
- Author:
Yan-Bing NIU
;
Xiao-Fei LEI
;
Lin-Yan SHEN
;
De-Fu WANG
;
Min YAO
;
Ping-Yi GUO
;
- Publication Type:Journal Article
- Keywords:
Recombinant PCR Fusion gene RNA silencing Virus resistance
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
MP-Rep fusion gene which size is about 1000bp was constructed by recombinant PCR technique.In order to construct fusion gene,Tobacco mosaic virus(TMV) partial movement protein gene(MP) and Cucumber mosaic virus(CMV) partial replicase gene(rep) were ligated.Two copies of MP-Rep fusion gene were ligated with soybean intron in inverted repeat manner,the recombinant fragments were then inserted into binary vector pBIN438 under the control of 35S promoter.Recombinant clone pBIN438-MP-Rep(i/r) which contained two different virus derived genes was constructed.Recombinant clone pBIN438-MP-Rep(i/r) in accord with expected design was certified by restriction endonuclease enzymes digestion and PCR analysis.This approach provides a basis for Broad-spectrum plant virus resistance mediated by RNA Silencing.
