Effects of Low-iodine Diet on the Expression of Homeobox Gene NKX-2.2 in Rat Cerebrum Tissue
- VernacularTitle:低碘膳食对大鼠脑组织中同源盒基因NKX-2.2表达的影响
- Author:
Rui ZHANG
;
Hai-Ze GE
;
Xiu-Jun ZHAO
;
Yuan LI
;
Gang GUO
;
- Publication Type:Journal Article
- Keywords:
Low-iodine Homeobox gene Real-time fluorescence quantitative PCR Brain development
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of low-iodine diet on the expression of homeobox gene NKX-2.2 in rat cerebrum tissue,and to explain the possible molecular mechanism of cerebrum development retardation caused by low-iodine.Methods:Female Wistar rats were randomly divided into two groups:Low-iodine group and control group,both fed with low-iodine feed,given the deionized water and KIO3 solution respectively,they were drawn from the 16-day pregnancy,new-born and 20th days old low-iodine and normal age offspring after three months,and detect the content of NKX-2.2 mRNA in the cerebrum tissue by Real-time fluorescence quantitative PCR techniques.Results:The thyroid hormone levels of low-iodine group in serum were significantly lower than the control group(P
