?C31 Integrase and Transgenic Animals Research
- VernacularTitle:?C31整合酶与转基因动物研制
- Author:
Li-Juan QU
;
Ying HUANG
;
- Publication Type:Journal Article
- Keywords:
?C31 integrase Transgenic animals Site-specific integration
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Streptomyces phage ?C31 integrase is a site-specific recombinase and introduce foreign gene to unidirectional recombination.Because of its integration mediated by ?C31 integrase without any cofactors and especially directed integration,it possesses high efficiency and stable expression of foreign gene.These advantages make it an attractive tool for genetic engineering.It has been widely used to transgene integration in mammalians and provides an opportunity to overcome the bottlenecks in the generation of transgenetic animals,such as random integration,low integral rate,low level of expression and so on.The ?C31 integrase will have a wide application in the near future.
