Polypyrimidine Tract Binding Protein Negatively Regulates the Expression of HBV Surface Antigen by Interacting with HBV Postranscriptional Regulatory Element
- VernacularTitle:多聚嘧啶序列结合蛋白(PTB)与HBV转录后调节元件(HPRE)结合抑制HBV表面抗原的基因表达
- Author:
Li-Ying CHENG
;
Xiao-Hua ZHANG
;
Yi LI
;
Xue-Fei CAI
;
Yuan HU
;
Ai-Long HUANG
;
Hua TANG
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus HBV posttranscriptional regulatory element(HPRE) Polypyrimidine tract binding protein(PTB) HBV surface antigen Gene expression
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to demonstrate PTB bind to HPRE,reverse transcription,PCR-mediated detection,were used.HepG2.2.15 cell line and HBs-HPRE transient expression cells were adopted to identify PTB function in HBV life cycle.The results showed that PTB could directly bind to HPRE RNA.Functional analysis indicated that PTB could inhibit the expression of HBs antigen and this inhibition was in a dose-dependent manner in HepG2.2.15 cells.Higher expression of HBs in cells transfected pcDNA3-HBs-HPRE comparing with pcDNA3-HBs,and this high expression could also be inhibited by PTB.The data demonstrated that PTB inhibits HBs expression by interacting with HPRE.
