The Comparative Analysis of Microorganism Diversity in Two Kinds of Packaged Chilled Beef on Sale
- VernacularTitle:两种包装市售冷却牛肉中微生物多样性的比较分析
- Author:
Zheng-Tang LI
;
Bai-Lin LI
;
Yong ZHAO
;
Jie OU
;
- Publication Type:Journal Article
- Keywords:
Chilled beef, 16S rDNA clone library analysis, PCR-DGGE, Microbial flora structure
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This research is for the purpose of comparative analysis of the microbial flora structure in the chilled beef with no packing and cling film, which under the same terms of sale. It was used the V3 area fragment of 16S rDNA to carry on PCR-DGGE, Meanwhile used the 16S rDNA sequence to analysis the microbial flora structure of the two samples, according to the technology of clone .The research discovered that the flora structure displays a biggish difference; there was 6 OTU in the chilled beef with cling film, mainly was that Lactococcus(28%), Lactobacillus (26%), Carnobacterium(18%) and Brochothrix (10%); but there was 18 OTU in the chilled beef with no packing, mainly was that Lactococcus(28%), Brchothrix(18%), Acinetobacter (11%). The result indicates that cling film played a certain inhibitory action regarding the Staphylococcus as well as the cold pole bacteria and such bacterium. And it can provide a certain theory ba-sis for the meat processing in the department of microorganism’s control.
