Prokaryotic Expression and Acetylation Assays of Histone Acetyltransferase PCAF
- VernacularTitle:组蛋白乙酰转移酶PCAF在原核细胞的表达及其生物学活性检测
- Author:
Wei-Zhong LI
;
Dan-Gui ZHANG
;
Jun ZENG
;
Ge-Fei WANG
;
Xiao-Xuan CHEN
;
You-Ying CHEN
;
Kang-Sheng LI
;
- Publication Type:Journal Article
- Keywords:
p300/CBP-associated factor Prokaryotic expression Acetylation assays
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
P300/CBP-associated factor(PCAF),an important member of histone acetyltransferase family(HATs) within eukaryotic cells,is capable of inducing the acetylation of histone,promoting the transcription of specific genes and involving in many biological effects.In the present study,full-length cDNA of PCAF was inserted into plasmid pGEX-5x-1,then the soluble protein GST-PCAF was expressed in E.coli BL21(DE3) after the optimization of inducing conditions.The recombinant protein was further purified with affinity chromatography and tested the activity by in vitro acetylation assays.High efficient PCAF protein produced by this method could serve for the study on the role of PCAF in gene regulation and the interaction between PCAF and other proteins.
