Expression of Inhibin in the Whole-body gamma-irradiated Mouse Ovary.
- Author:
Sang Soo KIM
;
Chang Joo LEE
;
Hyun Tae YOON
;
Yong Dal YOON
- Publication Type:Original Article
- Keywords:
Mouse ovary;
Follicle;
Inhibin;
Radiation
- MeSH:
Animals;
Female;
Follicular Atresia;
Immunohistochemistry;
Inhibins*;
Mice*;
Ovary*;
RNA, Messenger
- From:Korean Journal of Fertility and Sterility
2006;33(1):35-44
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purposes of the present study were to investigate the effect of gamma-radiation on the expression of inhibin-alpha proteins and genes for inhibin alpha, betaA, and betain the ovary. METHODS: Immature mice were whole-body gamma-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation,the ovaries were collected and used for immunohistochemistry for inhibin-alpha, and RT_PCR for inhibin-alpha, betaA, and betaB. RESULTS: The expression of the immunoreactive inhibins-alpha was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-alpha mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of betaA and betaB mRNAs. CONCLUSION: It might be thought that inhibin acts as one of the regulatory factors in the gamma-radiation-induced follicular atresia in mice
