Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis
- VernacularTitle:NASBA(依赖核酸序列的扩增)技术及其在病毒检测中的应用
- Author:
Shan-Dian GAO
;
Hui-Yun CHANG
;
Guo-Zheng CONG
;
Jun-Zheng DU
;
Jun-Jun SHAO
;
Tong LIN
;
- Publication Type:Journal Article
- Keywords:
Nucleic acid sequence-based amplification NASBA Isothermal amplification Viral diagnosis
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Nucleic acid sequence-based amplification(NASBA) is a sensitive,isothermal,transcription-based amplification system specifically designed for the detection of RNA targets,which could amplify templete RNA in 2h under isothermal condition at about 42?C and without any special equipment.NASBA is now widely applicated in diagnosis of many pathogenic microorganism.It is mainly about principles and applications of NASBA in viral diagnosis.
