Compare of Selectivity Enrichment Broth for Detectable Effect of Listeria monocytogenes
- VernacularTitle:选择性增菌液对单核增生性李斯特氏菌检出效果的比较
- Author:
Yang-Feng HU
;
Jun HAN
;
Ying-Min JIA
;
- Publication Type:Journal Article
- Keywords:
Food, Listeria monocytogenes, Selectivity enrichment broth
- From:
Microbiology
2008;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper investigated contamination situation of Listeria monocytogenes(Lm). To compare dif- ferent selectivity enrichment broth for detectable effect of Lm and compare detectable effect in different samples by using different methods, furthermore, choose the best enrichment broth for specific food. One hundred and thirty five random samples from raw meat, aquatic product, fruit and vegetable, quick-frozen food in Baoding. Applied LB enrichment broth, EB enrichment broth, new modification FDA enrichment broth and Fraser enrichment broth before separated by PALCAM selective agar, then identified by interna- tional standard method after PCR. Results: Four methods showed that there were 23 Lm positive, detected 5 Lm by LB method, 6 Lm by Fraser method, 5 Lm by EB method and 7 Lm by new modification FDA method. The total detectable rate of four methods had no large specificity, but to specific kind of food was different.
