Differentiation of Mouse Embryonic Stem Cells into Neural Cells on Silk Fibroin Scaffolds
- VernacularTitle:小鼠胚胎干细胞在丝素材料上向神经细胞分化的研究
- Author:
Liu-Jun BAO
;
Rong YE
;
Fu HAN
;
Jun-Chao ZHANG
;
Huan-Xiang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells Silk fibroin Neural cells
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Induced the mouse embryonic stem(ES)cells into neural cells on silk fibroin via the improved 4-/4+ RA method to explore the effect of the silk fibroin to the ES-derived neurons' growth,adherence and differentiation.Suspended the ES cells into EBs and then transferred them to three different substrates-coated 35 mm dishes including gelatin,Bombyx mori silk fibroin(SF) and Tussah silk fibroin(TSF) to identify the adherence and proportion of ES cells-derived neurons under these three substrates.The results showed that the EBs adhered to the gelatin and TSF are faster than to the SF.The average adhesive rate on gelatin and TSF are 90.3% and 84.4% respectively,and only 38.5% on SF,all the proportion of ?-Ⅲ-Tubulin positive cells is approximately 40%.It may provide important experimental information for tissue engineering,in which ES cells-derived neuron cells and silk fibroin materials are scaffolds,and also offer a source for cell therapy research of neurodegenerative disease.