Primary Study of Producing UMP by Overexpressing URA 5 and URA 3 Genes in Saccharomyces cerevisiae
- VernacularTitle:酿酒酵母中过表达URA 5及URA 3基因催化合成UMP的初步研究
- Author:
Gao-Xin LEI
;
Yong CHEN
;
Lin XU
;
Han-Jie YING
;
- Publication Type:Journal Article
- Keywords:
Orotate phosphoribosytransferase(OPRTase) Biocatalysis Saccharomyces cerevisiae Uridine 5′-monophosphate(UMP) Orotidine monophosphate decarboxylase(ODCase)
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
To increase the biotransfomation efficiency from the orotic acid to the uridine 5'-monophosphate(UMP),URA5 gene encoding orotate phosphoribosytransferase was amplified from Saccharomyces cerevisiae BY4742 by PCR,then it was inserted into the expression vector pYX212(contained orotidine monophosphate decarboxylase gene URA3)and the pYX212-URA5 was transformed into Saccharomyces cerevisiae BJX12 by electroporation.The recombinant strain was elementarily used to convert orotic acid to UMP.The results showed that pYX212-URA5/BJX12 could accumulate 7mmol/L UMP from 32mmol/L orotic acid in 26h,significantly higher than both control groups pYX212/BJX12(2.7mmol/L) and BJX12(2.4 mmol/L).
