Expression of Vitreoscilla Hemoglobin in Poly ?-glutamic Acid-producing Bacillus licheniformis WX-02
- VernacularTitle:透明颤菌血红蛋白在产聚?-谷氨酸地衣芽胞杆菌WX-02中的表达
- Author:
Huan XIONG
;
Xue-Tuan WEI
;
Zhi-Xia JI
;
Ming SUN
;
Shou-Wen CHEN
;
- Publication Type:Journal Article
- Keywords:
Bacillus licheniformis,Poly ?-glutamic acid,Vitreoscilla hemoglobin
- From:
Microbiology
2008;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Poly ?-glutamate is a biopolymer material that has a good application prospect.The Vitreoscilla hemoglobin(VHb) gene was integrated into the chromosome of Bacillus licheniformis WX-02 by integrative vector pDG1730-vgb.The expression of VHb was confirmed by CO-difference spectra analysis.It was shown by the results of batch cultures in a 3 L bioreactor that biomass and ?-PGA obtained in the recombinant M2 were 25.5 % and 20% higher than those of the control respectively.
