Comparison of Rhizospheric Soil DNA Isolation and Purification Methods from the Root of Fritillaria thunbergii Miq
- VernacularTitle:浙贝母根际土壤总DNA提取和纯化方法的比较
- Author:
Ying-Ying DOU
;
Zhan-Min LIN
;
Ying-De ZHU
;
Qun LU
;
Bo-Ping YE
;
- Publication Type:Journal Article
- Keywords:
F.thunbergii,Rhizospheric soil,Soil DNA,Extraction and purification,Method modification
- From:
Microbiology
2008;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Six DNA extraction methods and four DNA purification methods were compared and analyzed in this study to get higher quality DNA from the rhizospheric soil of Fritillaria thunbergii Miq.Results showed that higher purity DNA were harvested by pretreating the soil with 20 mmol/L EDTA(pH 7.5),then isolating soil DNA with CTAB-SDS-frozen-thawing,and further purified by agarose method.The recovery rate of this soil DNA was about 44.00 ?g/g ? 2.65 ?g/g soil,and they were qualified for the microbial diversity analysis in the rhizospheric soil of F.thunbergii Miq based on the 16S rDNA sequence.
