Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar
- VernacularTitle:一株家养野猪源猪圆环病毒2型的分离与鉴定
- Author:
Hai-Yan WANG
;
Song GAO
;
Xiu-Fan LIU
;
- Publication Type:Journal Article
- Keywords:
Domesticated wild boar,Porcine circovirus type 2,Isolation,Identification
- From:
Microbiology
2008;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
The nucleic acid fragment of porcine circovirus type 2(PCV2)was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome(PMWS).The sample were then inoculated into the Dulac cells and passaged for 8 generation.The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence.The sequence of genome was compared with other 9 strains originated from piglets in the same area.The result showed that these strains were in high homology.
