Design and Identification of Antisense Oligonucleotide Targeting to 16S rRNA of E.coli
- VernacularTitle:大肠杆菌16S rRNA的核酶设计和初步应用
- Author:
Jian-Ping MAO
;
Guo-Gang YUAN
;
Quan-Hui WANG
;
Wei WEI
;
Li-Jing WEI
;
Yu-Fang CUI
;
- Publication Type:Journal Article
- Keywords:
E.coli 16S rRNA Antisense oligonucleotide Accessible site MAST
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Targeting rRNA of bacteria is a new strategy for antibiotic agent development. The rRNA such as mRNA are naturally self-folded molecules which expose only limited accessible target-sites for binding. These accessible sites are pivotal for designing the effective antisense oligonucleotides, ribozymes, and DNAzymes. MAST, an RNA accessible site screening method, illustrated 6 accessible sites on 16S rRNA by immobilizing 16S rRNA and hybridizing with oligonucleotide library. 5 of the accessible sites were identified valid, and the antisense oligonucleotides targeted to which showed inhibition effectiveness on the proliferation. Among the 5 target sites, one showed the priority of accessibility. Ribozyme designed to this site showed obvious inhibition to the growth when induced expressing in the transfection E.coli.
