System of Intein-mediated PHB Purified Human Antimicrobial Peptide LL-37
- VernacularTitle:内含肽介导PHB纯化人源抗菌肽LL-37体系的构建
- Author:
Yin-Xia GU
;
Xue-Zhang ZHOU
;
Zhen-Wei SONG
;
Yu-Jiong WANG
;
- Publication Type:Journal Article
- Keywords:
Protein expression Poly-?-hydroxybutyrate (PHB) Containing peptides Human antibacterial peptide LL-37
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification.Choose human antibacterial peptide LL-37 as the purification objects,which is poison to prokaryotic cell.We construct intein-mediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations.
