The Current State and Trend of in Fed-Batch Mammalian Cell Culture
- VernacularTitle:动物细胞流加培养的技术现状和发展趋势
- Author:
Xing-Mao LIU
;
Zhao-Lie CHEN
;
- Publication Type:Journal Article
- Keywords:
Mammalian cells Fed-batch culture Medium Control strategy
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Fed-batch culture is the predominant mode of the current animal cell culture process for many recombinant protein production. Fed-batch culture operation is mainly based on the nutrient continuous consumption and demand of cells to design continuous or semi-continuous concentration feed medium to maintain or support high-density cell growth and improve volumetric productivity of target protein in the reactor. The main methods to improve production efficiency of fed-batch cells culture include the optimization of medium design, selection and optimization of feed strategy and regulation of cell metabolism.
