Targeted Gene Mutagenesis and Replacement Mediated by Zinc Finger Nucleases
- VernacularTitle:利用人工锌指蛋白核酸酶进行植物基因定点突变和置换
- Author:
Yu-Yang ZHANG
;
Xiao-Hui ZHANG
;
Chan-Juan ZHANG
;
- Publication Type:Journal Article
- Keywords:
Targeted mutagenesis Gene replacement Zinc finger nucleases Plant
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Gene targeted mutagenesis and replacement can be used to modify gene sequence in genomic background without position effect or insertion inactivation in transgenic plants. Targeted mutagenesis organism has little biosafety concerns free of transgenes or marker genes. Gene targeted mutagenesis and replacement in high plants now appears to be a potential tool for gene functional analysis in situ, crops genetic improvement and molecular design. Zinc finger nuclease(ZFN)is most important and would be widely used in gene targeted mutagenesis and replacement through introducing double-strand breaks in genome. Strategies for gene targeted mutagenesis and replacement in plants is discussed. ZFN is described in detail from its structure, operation model and application in plants. Developmental prospect of ZFN in plant gene targeted mutagenesis and replacement is also discussed.
