Mass Culture and Purification of Vero-based Influenza Virus
- VernacularTitle:Vero细胞流感病毒的大规模培养和纯化
- Author:
Chun-Yan HE
;
Ze CHEN
;
Shu-Jun WU
;
Li-Li TANG
;
Jie QIN
;
- Publication Type:Journal Article
- Keywords:
Vero cells Influenza virus Purification
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Establish mass-scale purification technology of cell-derived influenza virus. Methods: A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results: The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion: Providing a high effective purification technology for cell-derived influenza virus.
