The Influence of Thy-1.1 Stem Cell Transplantation on Neointimal Formation of Injured Artery
- VernacularTitle:Thy1.1干细胞移植能抑制动脉损伤后内膜增生
- Author:
Shao-Hong DONG
;
Hua-Dong LIU
;
Xin JIANG
;
- Publication Type:Journal Article
- Keywords:
Bone marrow stem cells;
Thy-1.1 antigen;
Restenosis;
Artery injury;
Rat
- From:
Chinese Journal of Hypertension
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Thy-1.1 stem cell transplantation on endothelial hyperplasia and restenosis.Methods Thirty 4-6 weeks male SD rats were sacrificed to obtain the Thy-1.1 stem cells.Carotid artery were injured by ballon in sixty female SD rat's were randomized to receive stem cell transplantation(5?10~6 Thy-1.1,n=30)or saline approach(n=30).About 5?10~6 Thy-1.1 stem cells were injected into the injured arter- y after carotid artery injury;while the control rats underwent carotid artery injury and was injected the same amount of saline.The animals were sacrificed,3,7,14,21 and 28 days after balloon denudation.The samples of carotid artery were harvested for pathological examination,RT-PCR and in situ hyhridzation(ISH)were used to detect the transplanted cells in the injured artery.Results The intimal thickness was thinner in stem cell transplantation group(I/M,Stem cell transplantation group:2.06?0.28 vs control group 2.42?0.19,P
