Stromal Interaction molecule 1 STIM1 Knockdown Inhibits Proliferation of Vascular Smooth Muscle Cells
- VernacularTitle:基质相互作用分子1基因敲除抑制血管平滑肌细胞增殖
- Author:
Rui-Wei GUO
;
Lan HUANG
;
Ming-Bao SONG
;
Hong WANG
;
Wei LI
;
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cell;
Stromal interaction molecule 1;
Cell proliferation;
Intracellu lar Ca~(2+);
RNAi
- From:
Chinese Journal of Hypertension
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of stromal interaction molecule 1(STIMI)on prohteration and intra- cellular Ca~(2+)change in vascular smooth muscle ceils(VSMC).Methods Rat VSMC were isolated from SD rats and primary cultured.Ad-si/rSTIM1 and Ad-hSTIM1 were transfected into VSMC.The protein of STIM1 was measured by Western blot,the proliferation of VSMC was analyzed by ~3H-thymidine(~3H-TdR)incorporation and cell count,the intracellular Ca~(2+)change was assessed By Aquaeosmos system.Ruselts Fourty-eight hours after transfection,as compared with Ad-hSTIMI group,the Ad-si/rSTIMI VSMC had lower expression of STIM1 protein (P
