The Effect of Activator Protein-1 Decoy Oligodeoxynucleotides on the Collagen Ⅰ and Ⅲ Expression in Rat Cardiac Fibroblast Cells Induced by Angiotension Ⅱ
- VernacularTitle:结合转录因子激活蛋白1的诱杀性寡聚脱氧核苷酸能抑制血管紧张素Ⅱ诱导的大鼠心肌成纤维细胞胶原表达
- Author:
Shuang-Lun XIE
;
Jing-Feng WANG
;
Rong-Qiong NIE
;
Wo-Liang YUAN
;
Fei LI
;
Mao-Huan LIN
;
- Publication Type:Journal Article
- Keywords:
Cardiac fibroblast;
Activator protein-1;
Decoy;
Angiotensin Ⅱ;
Collagen
- From:
Chinese Journal of Hypertension
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of activator protein-1(AP-1)decoy oligodeoxynucleotides (ODNs)on the myocardial fibrosis induced by angiotension Ⅱ(Ang Ⅱ)in vitro.Methods CFs of neonatal Spra- gue-Dawley(SD)rats were isolated by trypsin digestion method.CFs were co-cultured with 10~(-7)mol/L Ang Ⅱ in the presence of different concentration of activator protein-1(AP-1)decoy ODNs or mutational AP-1 decoy ODNs for 24 h.Collagen synthesis was assessed by hydroxyproline and the mRNA expression of collagen Ⅰ,collagen Ⅲ.Results The concentration of hydroxyproline increased significantly after treated by 10~(-7)mol/L Ang Ⅱ;decoy ODNs on the range of 10-200 nmol/L dose dependently decrease synthesis of collagen;Ang Ⅱ stimulates mRNA expression of collagen Ⅲ(1.04?0.07 vs 1.63?0.071,n=3,P
