Screening of Poly-?-glutamic Acid High Productive Strain and Optimization of Fermentation Medium
- VernacularTitle:聚?-谷氨酸高产菌的选育与培养基优化
- Author:
Xue-Chao WU
;
Xin-Jiang CAO
;
Zhi-Xia JI
;
Shou-Wen CHEN
;
- Publication Type:Journal Article
- Keywords:
Poly-?-glutamic acid, Bacillus subtilis, Mutation, Fermentation medium, Optimization
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Bacillus subtilis B6-1 was used as an original strain for mutagenic treatment and a defined medium was used as the selective medium. A mutant named B. subtilis W003 was isolated after three serial ultraviolet (UV) irradiations and one diethyl sulfate (DES) treatment. The ?-PGA yield on a rotary shaker was enhanced from 10.9 g/L in parental strain to 20.5 g/L in the mutant. It was illustrated by single factor experiments that the optimal carbon and nitrogen sources were glucose and (NH4)2SO4 respectively. The optimal fermentation medium was achieved by orthogonal test. In the optimal medium, a ?-PGA yield of 45.3 g/L was obtained after 36 h cultivation.
