Preliminary Study of Bio-transformation of Phytosterol by HPLC-MS
- VernacularTitle:高效液相色谱-质谱联用法分析微生物降解植物甾醇为雄甾烯酮
- Author:
Wei SHEN
;
Wei-Hong LIAO
;
Zhi-Ming RAO
;
Hui-Ying FANG
;
Jian ZHUGE
;
- Publication Type:Journal Article
- Keywords:
Phytosterol, Androst-4-end-3,17-dione, Androst -1,4-end-3,17-dion, Liquid chromatogra- phy-mass spectrometry (HPLC/MS)
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The method for analysis and determination the cleavage of soybean sterol, in which the soybean sterol was degraded and the products androst-1,4-diene-,17-dione (ADD) and androst-4-ene-3,17-dion (AD) were developed by Liquid Chromatography-mass Spectrometry. The HPLC conditions adopted were: a All- tima ODS-2 column (250 mm?4.6 mm, 5 ?m), a mobile phase consisted of menthanol-water (70:30), a flow rate of 1.0 mL/min, a room column temperature. and the detective wavelength was 244 nm.The ZMD Micromass electrospray ionization (ESI)-mass spectrometer was employed. In such conditions the corre- sponding HPLC chromatogram and MS spectrum were obtained. The method has a linear ranger of 0.01 mg/mL ~ 0.09 mg/mL, R2 =0.9999, the recoveries of ADD and AD were 102.6% and 105.90%, the RSD of ADD and AD were 3.02%, 3.5% and 3.08%, 3.24%. This method showed high sensitivity, accuracyand easy to perform. It is suitable to analysis the process cleavage of soybean sterol as well as quality control of product.
