Effect of Specific Growth Rate and Nitrogen Source on the Production of Recombinant Lateolabrax japonicus Growth Hormone by Pichia pastoris
- VernacularTitle:比生长速率和氮源对毕赤酵母生产重组鲈鱼生长激素的影响
- Author:
Chun WEI
;
Xiang-Shan ZHOU
;
Yuan-Xing ZHANG
;
- Publication Type:Journal Article
- Keywords:
Pichia pastoris, Intracellular production, Exponential feeding, Specific growth rate
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The bioreactor production of recombinant Lateolabrax japonicus growth hormone (rljGH) expressed intracellularly by Pichia pastoris was investigated. A strategy of feeding methanol at the exponential rate was established and the effect of specific growth rate on the rljGH production was examined. The results indicated that the average specific production rate increased and the rljGH production duration decreased as the specific growth rate increased. The maximum specific rljGH production (0.58 mg/g WCW) was achieved at a specific growth rate of 0.029/h. The effect of supplementing ammonium sulfate, peptone and yeast ex- tract on the rljGH production was further investigated. The results indicated that the effects of ammonium sulfate and peptone were not significant. Supplementing yeast extract of 2.5 g/L was advantageous for the rljGH production. The duration of the rljGH production was increased to 23 h from 17 h and the fermenta- tion stability of run-to-run could be improved.
