Expression and Characterization of Thrombin Inhibitor TTI
- VernacularTitle:凝血酶抑制剂TTI的表达及性质研究
- Author:
Li-Ping XIE
;
Ke SHANG
;
Chun-Bao ZHU
;
Bao-Quan ZHU
;
You-Jia HU
;
- Publication Type:Journal Article
- Keywords:
TTI Thrombin inhibitor Inhibitory
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
TTI gene coding for Tsetse thrombin inhibitor was modified with E.coli bias codon and expressed in Escherichia coli with high efficiency.Recombinant protein was purified to more than 98% purity.Assay for enzyme activity determination was set up.The result showed that the fusion protein exhibited inhibiting activity for thrombin.Inhibitory rate of purified TTI was 73% when concentration of thrombin and substrate was 10U/ml and 250?mol/L respectively.Inhibition pattern was determined as competitive with Ki at 35?mol/L.
