Cardioprotective Effects of Granulocyte Colony-Stimulating Factor in Mice Cardiac Hypertrophy Induced by Angiotensin Ⅱ
- VernacularTitle:粒细胞集落刺激因子对于血管紧张素Ⅱ所致小鼠心室肥厚的保护作用
- Author:
Nan JIA
;
Qiu-Ping HUANG
;
Wei JIN
;
Jian-Jun ZHANG
;
Qiu-Yan DAI
;
Shao-Wen LIU
;
- Publication Type:Journal Article
- Keywords:
Granulocyte colony-stimulating factor;
Angiotensin Ⅱ;
Cardiac hypertrophy;
Angiotensin converting enzyme
- From:
Chinese Journal of Hypertension
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Background Granulocyte colony-stimulating factor(G-CSF)has been reported to have beneficial effect on cardiac dysfunction in post infarction and doxorubicin-induced cardiomyopathy.Objective To investigate the effects of G-CSF on cardiac remodeling in cardiac hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ).Methods Thirty-six male wild type mice(WT)were allocated randomly to receive subcutaneously G-CSF(10 ?g/kg per day, n=9),or Ang Ⅱ(2.88 mg/kg per day,n=9),or Ang Ⅱ plus G-CSF(Ang Ⅱ 2.88 mg/kg+G-CSF 10 ?g/kg,n =9)for 4 weeks with untreated WT(n=9)as controls.Blood pressure and cardiac function were measured. Heart weight/body weight ratio,myocyte cross-sectional area and fibrosis area were determined.The mRNA ex- pression of osteopontin(OPN)in myocardium was detected by RT-PCR.The expressions of angiotensin converting enzyme(ACE),ACE2 and phosph-p70S6 kinase protein in myocardium were assessed by Western-Blot.Results Ang Ⅱ significantly elevated blood pressure(SBP,Ang Ⅱ:139.7?1.6 vs WT:108.7?2.3 mmHg,P0.05),but significantly attenuated the myocyte cross-sectional area(Ang Ⅱ+G-CSF:181.06?0.11 vs Ang Ⅱ:202.02?0.16 ?m~2,P
