Screening, Identifying and Function Analysis of Polyketide Synthase I Cluster from the Environmental Strain X-2 Which Produce Macrolactins
- VernacularTitle:产Macrolactins的海洋细菌X-2中Ⅰ型PKS基因簇的筛选鉴定与功能分析
- Author:
Xiao-Yi DONG
;
Liang-Hua WANG
;
Ming-Juan SUN
;
Ying ZONG
;
Yu-Liang JIAO
;
Bing-Hua JIAO
;
- Publication Type:Journal Article
- Keywords:
Macrolactin, Polyketide synthase, Bacillus sp.
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Macrolactins are 24-membered macrolides produced by unidentified marine bacterium, Actinomadura sp. and Bacillus sp., which exhibit both antibacterial and antitumor activities in vitro. The environmental strain X-2 which was isolated from the sediment of the East China Sea produce Macrolatin A, B and O. In this study, a set of degenerate oligonucleotide primers, designed for amplification ketosynthase(KS) domains, had been employed to identify KS gene fragments of the X-2 DNA samples. One 645 bp KS fragment(GenBank accession no. EF486351)had been cloned and used as a probe to screen the genome DNA fosmid library of X-2. Three positive clones were selected and sequenced, Homologous analysis and the function prediction of the obtained PKS gene fragments suggested that macrolactin is the Polyketide Biosynthesis Product of the gene cluster obtained in the environmental strain X-2.
