Preliminary Study on Solid-state Fermentation of Streptomyces Strain Men-myco-93-63
- VernacularTitle:链霉菌Men-myco-93-63固体发酵的初步研究
- Author:
Chun-Ying HAO
;
Qing-Fang MENG
;
Guo-Tong CHI
;
Wen-Xiang YANG
;
Da-Qun LIU
;
- Publication Type:Journal Article
- Keywords:
Streptomyces Men-myco-93-63, Solid-state fermentation, Culture medium, Cultural conditions
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The culture medium and cultural conditions of solid-state fermentation of Streptomyces Menmyco-93-63 were tested in this study. The suitable medium which contains rice, sorghum, millet bran, and rice hull with the proportion of 2:2:3:3 was developed for the spore production of Streptomyces Men-myco-93-63 using single substrate screening, mixture substrate screening and orthogonal experiments, and the sporulation was up to 2.52?109 CFU/g. And then, initial charge, initial ratio of water to solid, inoculating quantity, and culture temperature impact to sporulation of Streptomyces Men-myco-93-63 were tested. The favorite cultural conditions are developed as the following: the initial charge is 15 g in 500 mL Erlenmeyer flask; initial ratio of water to solid is 1.7:1.0 (V/W, rice hull excluding), inoculating quantity is 7 mL, culture temperature is 28℃.
