Isolation and Purification of Antibiotic from the Fermentation Broth of Micromonospora carbonacea JXNU-1 and Its Physical-chemical Properties
- VernacularTitle:炭样小单孢菌JXNU-1广谱抗生素产物的分离及其理化性质
- Author:
Zhong-Er LONG
;
Yue-Jin ZHU
;
Yun-Hong HUANG
;
Xue-Qin FU
;
- Publication Type:Journal Article
- Keywords:
Micromonospora carbonacea, Antibiotics, Isolation and purification
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the strain of Micromonospora carbonacea JXNU-1 with board-spectrum antimicrobial activity, the technology for the isolation and purification of antibiotic from the fermentation broth of the Micromonospora carbonacea, and its physical-chemical properties were studied. The results showed that, the antibiotic was stable under the condition of high temperature and alkali, but not in acid solution. After the pretreatment of centrifugation and filtration to remove the cells and lipids, the antibiotic was absorbed to negative exchange resin, and the impurity was excluded when 2 mol/L NaCl was used as primary eluent. The antibiotic could be eluted with 20% alcohol as eluent, and the eluting speed of the antibiotic was greatly accelerated as 2 mol/L NaCl was added into 20% alcohol as final eluent. Aqueous solution of the antibiotic was yielded from the alcohol-salt eluant by decompression concentration to wipe off alcohol and by dialysis to exclude salt. One active component was detected in antibiotic solution by paper chromatography, and theHPLC purity was over 99%. As the antibiotic shows positive color-forming reaction to Molish reagents, Benedict’s reagents and Diohenvlamine reagents, combined with the characteristics of absorption spectra, it is deduced that the antibiotic belongs to nucleoside antibiotics.
